LOCUS       sequence-76992-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat5
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    38..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             77..99
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             110..154
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             170..187
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
       61 cccctctaga aataattttg tttaacttta agaaggagat atacatatga aagaaaccgc
      121 tgctgctaaa ttcgaacgcc agcacatgga cagcccagat ctgggtaccc tggtgccacg
      181 cggttccatg gcgatatcgg atcgggaagg tgtcaacgac aacgaagaag gctttttcag
      241 cgcccgtgca gcaatcgcag cggctcaggc aaacccgaac gccaaactgg gtagcaactc
      301 tctggccgaa ctggtggttt ttggtcgtct gccggttgaa ttcgtcccgg tgcgtcatgt
      361 cctgctgctg agccgcaccg attggcagat tatctctgaa atcgcgacgc gcgcggccga
      421 acaatacgtt atcgacgaat acaacaaggg cgccgtggca agtctgacct ccgttgctaa
      481 gtatccgcag ctgaccatcc gtacgtggtt tgaactggcg ccgaaagcag ctaatattat
      541 cgttaaggtc ctggcttttg cgcaaagctt cattggtcgt gcagcagcac tggctgcagc
      601 agatgctcgt ctgctggcgt ggctggaaac cctgaaagca ccgccggctc cgccggcacc
      661 ggcacgtgct ctgcatctgg tggataacac cgacattgca cgcatcacgt ttattgatgg
      721 cgacgaaggt atcctgctgc accgctatag ta
//
