LOCUS       sequence-76997-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ATG8 fused at the N terminus to GFP under the control of 
            the CUP1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Wang K, Yang Z, Liu X, Mao K, Nair U, Klionsky DJ
  TITLE     Phosphatidylinositol 4-kinases are required for autophagic membrane 
            trafficking.
  JOURNAL   J Biol Chem. 2012 Nov 2;287(45):37964-72. doi: 
            10.1074/jbc.M112.371591. Epub 2012 Sep 13.
  PUBMED    22977244
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.371591"; journalName: "J Biol Chem"; date: 
            "2012-11-2- 2"; volume: "287"; issue: "45"; pages: "37964-72"
COMMENT     Sequence Label: CuGFP-ATG8(416)
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    35..537
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        574..678
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggttaatgtc atgataataa tggtttctta gacggatcgc ttgcctgtaa cttacacgcg
       61 cctcgtatct tttaatgatg gaataatttg ggaatttatc tgtgtttatt tatttttatg
      121 ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa
      181 aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat
      241 tagacaagaa aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa
      301 tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata
      361 cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt
      421 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt
      481 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa
      541 aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      601 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      661 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      721 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      781 gaaga
//
