LOCUS       sequence-77001-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ATG8 fused at the N terminus to GFP under the control of 
            the CUP1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Wang K, Yang Z, Liu X, Mao K, Nair U, Klionsky DJ
  TITLE     Phosphatidylinositol 4-kinases are required for autophagic membrane 
            trafficking.
  JOURNAL   J Biol Chem. 2012 Nov 2;287(45):37964-72. doi: 
            10.1074/jbc.M112.371591. Epub 2012 Sep 13.
  PUBMED    22977244
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.371591"; journalName: "J Biol Chem"; date: 
            "2012-11-2- 2"; volume: "287"; issue: "45"; pages: "37964-72"
COMMENT     Sequence Label: CuGFP-ATG8(416)
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..270
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(315..343)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctcctagt tagaaaaaga catttttgct
       61 gtcagtcact gtcaagagat tcttttgctg gcatttcttc tagaagcaaa aagagcgatg
      121 cgtcttttcc gctgaaccgt tccagcaaaa aagactacca acgcaatatg gattgtcaga
      181 atcgtataaa agagaagcaa ataactcctt gtcttgtatc aattgcatta tagtatcttc
      241 ttgttagtgc aatatcatat agaagtcatc gactagtgga tcctatgagt aaaggagaag
      301 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      361 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      421 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcgcgt
      481 atggtcttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      541 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      601 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      661 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      721 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      781 aaattagaca caacattgaa gatgg
//
