LOCUS       sequence-77003-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat12
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..189
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             205..222
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatgaaagaa accgctgctg ctaaattcga acgccagcac
      181 atggacagcc cagatctggg taccctggtg ccacgcggtt ccatggcgat atcggatcgg
      241 gaaggtgtga atgataatga agaaggcttt ttctctgctc gtgttaccga cgccgaaatc
      301 gctgaagtcc tggctcgctt tggcctgatc ccggaattta ttggtcgtgc cgaaacgctg
      361 tattacattg tcaaagtggg caacctggca tttctggatg tgaccggtcg cctgctggaa
      421 ctgaccttca cggaacagac cacgaaagcg gaaggcgccc tgctgatcaa cgcggttggt
      481 gtcgatgacg tgaagaccct ggccgaaggc cagaatgttg aatttgaaat tcaggatggt
      541 caaaaactgg aagaagatga cgtggcgacc ccgggcgaag gtcaagttct gctgcgtgtg
      601 gttggtgtcg caggcggtgc tgaaaaagcg ctgggcgcca acctggttct gaccgaaggt
      661 gccaagatcc agggcattgg tgcaggcttc atcccggcta atctggatct gaaagaaggc
      721 gacgccgttc aactggtcgg ttttggcacc ttcaaaattg cggccgcaaa cgtcccggca
      781 tttgtgagtg gcaagtattg ggatgtggaa ctgcgtctgc agggtatcgc
//
