LOCUS       sequence-77006-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 6, small linker insert, final 
            entry vector for the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_6'_Linker
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..57
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     39..56
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          154..726
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 ggaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaggt
       61 ctcatcaggc ggatccggcg gcggtaccta agtgagaccc tcatcctccc gggtaactaa
      121 gtaaggatct cgaccgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct
      181 ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg
      241 tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt
      301 cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact
      361 ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg
      421 tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat
      481 tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt
      541 ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg
      601 gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata
      661 aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc
      721 ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaatta
      781 attcctcgac ggatcgta
//
