LOCUS       sequence-77009-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 3, LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_3_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    172..193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(208..213,214..231,232..238)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    246..262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     251..273
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     270..286
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     270..286
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             282..665
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        304..322
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      416..425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      420..429
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(459..478)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(460..478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(485..502)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(485..501)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(494..516)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(703..721)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ggtctcatag tggtaccagc ggtggcgtgg cgagcgcagc gagtcagtga gcgaggaagc
       61 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      121 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      181 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      241 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      301 gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg ggagctctcc
      361 catatggtcg acctgcaggc ggccgcgaat tcactagtga tatcgaattc ccgcggccgc
      421 catggcggcc gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag tcgtattaca
      481 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      541 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      601 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
      661 attaagcgcg ccacgccacc gctggatcca gaggttgaga cctctatagt gtcacctaaa
      721 tcaagcttgc tccgacgtcc ccaggcagaa tggcggttcc ctaaacgagc attgcttata
      781 tagacctccc att
//
