LOCUS       sequence-77011-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ATG8 fused at the N terminus to GFP under the control of 
            the endogenous promoter in the pRS416 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Guan J, Stromhaug PE, George MD, Habibzadegah-Tari P, Bevan A, Dunn 
            WA Jr, Klionsky DJ
  TITLE     Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, 
            pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia 
            pastoris.
  JOURNAL   Mol Biol Cell. 2001 Dec;12(12):3821-38.
  PUBMED    11739783
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2001-12"; volume: "12(12)"; pages: "3821-38"
COMMENT     Sequence Label: GFP-ATG8(416)/GFP-AUT7(416)
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..527
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        564..668
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgataataat ggtttcttag acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt
       61 ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt
      121 ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa
      181 caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa
      241 aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt
      301 ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca
      361 ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc
      421 ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt taatttatat
      481 atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa aaggacccag
      541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      661 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      721 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatca
//
