LOCUS       sequence-77013-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 5, LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_5_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    240..261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(276..281,282..299,300..306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    314..330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     319..341
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     338..354
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     338..354
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             350..733
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        372..390
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      484..493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      488..497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(527..546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(528..546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(553..570)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(553..569)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(562..584)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(771..789)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gtccccaaac tcaccctgaa gttctcagga tcggtcgaga tccttactta gttacccggg
       61 aggatgaggg tctcactgag gtaccagcgg tggcgtggcg agcgcagcga gtcagtgagc
      121 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      181 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      241 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      301 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      361 tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg
      421 agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgata tcgaattccc
      481 gcggccgcca tggcggccgg gagcatgcga cgtcgggccc aattcgccct atagtgagtc
      541 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      601 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      661 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
      721 agcggcgcat taagcgcgcc acgccaccgc tggatcccag cagtgagacc tctatagtgt
      781 cacctaaatc aagcttgctc cgacgtcccc aggcagaatg gcggttccct aaacgagcat
      841 tgcttatata gacctcccat t
//
