LOCUS       sequence-77014-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 7, LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_7_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..58
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    175..196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(211..216,217..234,235..241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    249..265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     254..276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     273..289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     273..289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             285..668
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        307..325
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      419..428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      423..432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(462..481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(463..481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(488..505)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(488..504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(497..519)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(706..724)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gagggtctca cggcggtacc agcggtggcg tggcgagcgc agcgagtcag tgagcgagga
       61 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      121 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      181 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      241 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      301 caagctattt aggtgacact atagaatact caagctatgc atccaacgcg ttgggagctc
      361 tcccatatgg tcgacctgca ggcggccgcg aattcactag tgatatcgaa ttcccgcggc
      421 cgccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt
      481 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      541 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      601 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
      661 cgcattaagc gcgccacgcc accgctggat ccgcaacttg agacctctat agtgtcacct
      721 aaatcaagct tgctccgacg tccccaggca gaatggcggt tccctaaacg agcattgctt
      781 atatagacct cccatta
//
