LOCUS       sequence-77015-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for use in AdEasy System. Used for expression of 
            transgenes under a CMV promoter when no GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pShuttle-CMV
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(127..507)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..210)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     401..420
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   545..647
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
ORIGIN
        1 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
       61 tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca ttttttaacc
      121 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
      181 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
      241 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
      301 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
      361 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
      421 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
      481 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat taattcttaa
      541 ttaacatcat caataatata ccttattttg gattgaagcc aatatgataa tgagggggtg
      601 gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag tgtggcggaa
      661 gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa aagtgacgtt
      721 tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta ggcggatgtt
      781 gtagtaaatt tgggcgtaac c
//
