LOCUS       sequence-77016-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo GFP based system to measure break induced replication (BIR) 
            repair efficiency. expression of I-SceI generates a double strand 
            break that when repaired by BIR mechanism will restore GFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  TITLE     pBIR-GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBIR-GFP
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    135..216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(172..191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     226..245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     583..602
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    601..656
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     702..721
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
       61 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      121 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      241 tcatcaatgt ataagcttgg gctgcaggtc gagggatctc cataagagaa gagggacagc
      301 tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat gtaaggaaaa
      361 ttttagggat gttaaagaaa aaaataacac aaaacaaaat ataaaaaaaa tctaacctca
      421 agtcaaggct tttctatgga ataaggaatg gacagcaggg ggctgtttca tatactgatg
      481 acctctttat agccaacctt tgttcatggc agccagcata tgggcatatg ttgccaaact
      541 ctaaaccaaa tactcattct gatgttttaa atgatttgcc ctcccatatg tccttccgag
      601 tgagagacac aaaaaattcc aacacactat tgcaatgaaa ataaatttcc tttattagcc
      661 agaagtcaga tgctcaaggg gcttcatgat gtccccataa tttttggcag agggaaaaag
      721 atctcagtgg tatttgtgag ccagggcatt ggccacacca gccaccacct tctg
//
