LOCUS       sequence-77018-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 2, LacZ, final entry vector for
            the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_2'_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    241..262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(277..282,283..300,301..307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    315..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     320..342
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     339..355
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     339..355
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             351..734
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        373..391
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      489..498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(528..547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(529..547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(554..571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(554..570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(772..790)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ggtccccaaa ctcaccctga agttctcagg atcggtcgag atccttactt agttacccgg
       61 gaggatgagg gtctcactta ggtaccagcg gtggcgtggc gagcgcagcg agtcagtgag
      121 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      181 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      241 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      301 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      361 ttacgccaag ctatttaggt gacactatag aatactcaag ctatgcatcc aacgcgttgg
      421 gagctctccc atatggtcga cctgcaggcg gccgcgaatt cactagtgat atcgaattcc
      481 cgcggccgcc atggcggccg ggagcatgcg acgtcgggcc caattcgccc tatagtgagt
      541 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      601 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      661 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
      721 tagcggcgca ttaagcgcgc cacgccaccg ctggatccgg agcctgagac ctctatagtg
      781 tcacctaaat caagcttgct ccgacgtccc caggcagaat ggcggttccc taaacgagca
      841 ttgcttatat agacctccca ttaggcacgc ctaccgccca tttacgtcaa tggaacgccc
      901 atttgcgtca ttgcccctcc ccattgacgt caatggggat gtacttggca gccatcgcgg
      961 g
//
