LOCUS       sequence-77025-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo GFP based system to measure break induced replication (BIR) 
            repair efficiency. expression of I-SceI generates a double strand 
            break that when repaired by BIR mechanism will restore GFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     pBIR-GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBIR-GFP
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(238..750,751..753,754..756))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     431..450
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      753..762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctcgtggg atcattgttt ttctcttgat tcccactttg tggttctaag tactgtggtt
       61 tccaaatgtg tcagtttcat agcctgaaga acgagatcag cagcctctgt tccacataca
      121 cttcattctc agtattgttt tgccaagttc taattccatc agaaggttca gattaacgct
      181 tacaatttac gccttaagat taccctgtta tccctaggcg agctgcacgc tgccgtcctc
      241 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
      301 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      361 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      421 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
      481 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
      541 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
      601 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      661 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      721 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatccacgcc
      781 g
//
