LOCUS       sequence-77026-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 7, LacZ, final entry vector for
            the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_7'_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..81
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    198..219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(234..239,240..257,258..264)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    272..288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     277..299
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     296..312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     296..312
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             308..691
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        330..348
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      442..451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      446..455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(485..504)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(486..504)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(511..528)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(511..527)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(520..542)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(729..747)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 cttacttagt tacccgggag gatgagggtc tcacttaggt accagcggtg gcgtggcgag
       61 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
      121 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      181 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      241 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      301 aacagctatg accatgatta cgccaagcta tttaggtgac actatagaat actcaagcta
      361 tgcatccaac gcgttgggag ctctcccata tggtcgacct gcaggcggcc gcgaattcac
      421 tagtgatatc gaattcccgc ggccgccatg gcggccggga gcatgcgacg tcgggcccaa
      481 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
      541 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      601 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      661 cgaatggacg cgccctgtag cggcgcatta agcgcgccac gccaccgctg gatccgcaac
      721 ttgagacctc tatagtgtca cctaaatcaa gcttgctccg acgtccccag gcagaatggc
      781 ggttccctaa acgagcattg cttatataga cctcccatta ggcacgccta ccgcccattt
      841 acgtcaatgg aacgcccatt tgcgtcattg cccctcccca ttgacgtcaa tgggg
//
