LOCUS       sequence-77031-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Golden Gate assembly; middle entry vector for
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pME_GGWDest+
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..193
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     220..240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        222..240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     277..293
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     277..293
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(327..343)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(328..344)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     366..383
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    500..521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(536..541,542..559,560..566)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    574..590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     579..601
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     598..614
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     598..614
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        632..650
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     632..649
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      744..753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(787..806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(788..806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(813..830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(813..829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(822..844)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atcaagggtc cccaaactca ccctgaagtt ctcaggatcg gtcgagatcc ttacttagtt
       61 acccgggagg atgaggggat atcagctgga tggcaaataa tgattttatt ttgactgata
      121 gtgacctgtt cgttgcaaca aattgataag caatgctttc ttataatgcc aactttgtac
      181 aagaaagctg ggtgctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      241 gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg
      301 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagcctta agagaccccg
      361 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      421 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      481 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      541 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      601 gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc
      661 tatgcatcca acgcgttggg agctctccca tatggtcgac ctgcaggcgg ccgcgaattc
      721 actagtgata tcgaattccc gcggccgcca tggcggccgg gagcatgcga cgtcgggccc
      781 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
      841 tgggaaaacc ctggcgttac c
//
