LOCUS       sequence-77032-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for 
            measurement of cotranslational protein degradation, use with 50572.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Turner GC, Varshavsky A
  TITLE     Detecting and measuring cotranslational protein degradation in vivo.
  JOURNAL   Science. 2000 Sep 22;289(5487):2117-20.
  PUBMED    11000112
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-09-22"; volume: "289(5487)"; pages: "2117-20"
COMMENT     Sequence Label: pDBD-R
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..266)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     232..250
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(276..503)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(531..557)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggcgattggg taccggccgc aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa
       61 ggttttcagt ataatgttac atgcgtacac gcgtctgtac agaaaaaaaa gaaaaatttg
      121 aaatataaat aacgttctta atactaacat aactataaaa aaataaatag ggacctagac
      181 ttcaggttgt ctaactcctt ccttttcggt tagagcggat gtggggggag ggcgtgaatg
      241 taagcgtgac ataactaatt acatgactcg agttaaccac ctcttagcct tagcacaaga
      301 tgtaaggtgg actccttctg aatgttgtaa tcagacagcg ttctaccgtc ttctagctgc
      361 ctaccggcaa agatcaatct ttgttgatct ggagggatac cttccttgtc ttgaattttc
      421 gacttaacgt tgtcgatggt atcggaagat tcaacttcca atgttatggt tttaccggtc
      481 aaagtcttga cgaaaatctg catggtcgac ccactacccg ctaaaaatgc agcgtaatct
      541 ggaacatcgt atgggtaggt accgtctttc ttctcgtaga cttcaaactt atacttgatg
      601 cctttttcct cctggacctc agagaggacg cctgggtatt ctgggagaag tttatacttc
      661 cccaaatcaa tttctgggaa aaacgtgtca ctttcaaatt cctgcatgat ccttgtcaca
      721 aagagtctga ggtggcctgg ttgattcatg gcttcctggt aaacagaact gcctccgact
      781 atccaaacca tgtctacttt acttgccaat tccggttgtt caataagtct taa
//
