LOCUS       sequence-77040-e        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRb01_syn20
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..135
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(255..257,258..971)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             255..968
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(285..313)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     908..928
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
       61 cagctggcaa ttccgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      121 ggcgtatcac gaggcccttg cgcggataaa aatttcattt gcccgtgaaa catttcccgc
      181 ccatctaccg tatatgccat cagctgcgtt catcagcgag gatcctgtca atttccgcga
      241 tagaggaggt aaagatgagc aaaggagaag aacttttcac tggagttgtc ccaattcttg
      301 ttgaattaga tggtgatgtt aatgggcaca aattttctgt ccgtggagag ggtgaaggtg
      361 atgctacaaa cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc
      421 cgtggccaac acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg
      481 atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
      541 gcactatatc tttcaaagat gacgggacct acaagacgcg tgctgaagtc aagtttgaag
      601 gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca
      661 ttcttggaca caaactcgag tacaacttta actcacacaa tgtatacatc acggcagaca
      721 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacgttgaa gatggttccg
      781 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      841 cagacaacca ttacctgtcg acacaatctg tcctttcgaa agatcccaac gaaaagcgtg
      901 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
      961 tctacaaata ataaatgtcc agacctgcag gcatgcaagc ctctagaggc at
//
