LOCUS       sequence-77042-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRb02_2817
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(253..255,256..969)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             253..966
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(283..311)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     906..926
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
       61 gctggcaatt ccgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      121 cgtatcacga ggcccttcat gacaaacaaa aacagatgcg ttacggaact ttacaaaaac
      181 gagacactct aaccctttgc ttgctcaaat tgcagctgga tcctgtcaat ttccgcgata
      241 gaggaggtaa agatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      301 gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat
      361 gctacaaacg gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg
      421 tggccaacac ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat
      481 cacatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc
      541 actatatctt tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt
      601 gatacccttg ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt
      661 cttggacaca aactcgagta caactttaac tcacacaatg tatacatcac ggcagacaaa
      721 caaaagaatg gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt
      781 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      841 gacaaccatt acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac
      901 cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc
      961 tacaaataat aaatgtccag acctgcaggc atgcaagc
//
