LOCUS       sequence-77049-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRb15_up436
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..136
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(256..258,259..972)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             256..969
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(286..314)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     909..929
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
       61 ccagctggca attccgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
      121 aggcgtatca cgaggccctt cagtacaaaa ttttttagat gcgttcttgg gaaccgaacg
      181 ccggtgcccg cgttcggttc cggggatctt atcaactttt ggatcctgtc aatttccgcg
      241 atagaggagg taaagatgag caaaggagaa gaacttttca ctggagttgt cccaattctt
      301 gttgaattag atggtgatgt taatgggcac aaattttctg tccgtggaga gggtgaaggt
      361 gatgctacaa acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt
      421 ccgtggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc ccgttatccg
      481 gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      541 cgcactatat ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt caagtttgaa
      601 ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga agatggaaac
      661 attcttggac acaaactcga gtacaacttt aactcacaca atgtatacat cacggcagac
      721 aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacgttga agatggttcc
      781 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      841 ccagacaacc attacctgtc gacacaatct gtcctttcga aagatcccaa cgaaaagcgt
      901 gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag
      961 ctctacaaat aataaatgtc cagacctgca ggcatgcaag
//
