LOCUS       sequence-7705-ex       10112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Young BA, Taooka Y, Liu S, Askins KJ, Yokosaki Y, Thomas SM, 
            Sheppard D
  TITLE     The cytoplasmic domain of the integrin alpha9 subunit requires the 
            adaptor protein paxillin to inhibit cell spreading but promotes cell
            migration in a paxillin-independent manner.
  JOURNAL   Mol Biol Cell. 2001 Oct . 12(10):3214-25.
  PUBMED    11598204
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2001 Oct . 12(10):3214-25."
COMMENT     Sequence Label: integrin alpha9 / alpha2 pcDNA1 neo
FEATURES             Location/Qualifiers
     source          1..10112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            join(1204..1278,1279..1363,1364..1383)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     enhancer        1542..1921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1922..2125
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2076..2096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2170..2189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2170..2188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5448..5466)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5449..5466)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          5550..5615
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5745..5765
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6037..6171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6199..6218)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6495..6757
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             7117..7911
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7171..7190)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7781..7800
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          8332..8397
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8527..8547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8972..9106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9953..10088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     1201 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     1261 agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     1321 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     1381 aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     1441 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     1501 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     1561 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     1621 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1681 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1741 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1801 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1861 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1921 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1981 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2041 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2101 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     2161 tatcgaaatt aatacgactc actataggga gacccaagct tagagcgggc gctatgggga
     2221 gccggacgcc agagtcccct ctccatgccg tgcagctgcg ctggggcccc cggcgccgac
     2281 ccccgctact gccgctgcta ttgctgctcg tgccgccgcc accgcgggtc gggggctaca
     2341 acctggatcc gcagcgcccc gtgcacttcc agggccccgc tgactcgttc ttcggctacg
     2401 cagttctgga gcatttccac gacaacacgc gctgggtcct tgtgggcgca ccaaaggcag
     2461 attccaaata cagcccttca gtgaagtctc ctggggctgt gtttaagtgc cgtgttcaca
     2521 ccaaccctga ccggagatgc accgaactgg acatggctcg agggaagaat cggggcacgt
     2581 cctgcggaaa gacctgccgg gaagaccgcg atgatgagtg gatgggggtg agcctggccc
     2641 gacagcccaa ggctgatggc cgtgtgttgg cctgtgctca tcgctggaag aacatctact
     2701 atgaagccga ccacatccta ccccatggct tctgctacat catcccctcc aacctccagg
     2761 ccaaaggcag gacgctgatc ccttgctatg aagagtataa gaagaagtac ggagaggaac
     2821 acggctcctg ccaggctggg atagcgggct tcttcaccga ggagctggtg gtgatgggtg
     2881 ctccagggtc attttattgg gctggaacca tcaaagtgct gaaccttacg gacaacacct
     2941 atttaaaact gaacgacgaa gtgatcatga acaggcggta cacctacctg ggctacgcag
     3001 tgaccgctgg ccacttctct cacccgtcca ccattgatgt ggtaggaggt gccccacagg
     3061 acaaaggcat cggcaaggtt tatattttca gagctgaccg aagatcaggc accttaatta
     3121 agatctttca agcatcaggt aaaaagatgg gctcttactt cggctcctcc ttgtgcgcag
     3181 ttgacctgaa tggggacggc ctctctgacc tgctggtggg ggcccccatg ttttctgaga
     3241 tcagggatga gggacaggtc actgtctaca tcaacagagg aaatggagcc ctcgaggagc
     3301 agctggctct gactggggat ggtgcctaca atgcccactt tggagagagc attgccagcc
     3361 tggacgatct ggacaatgat gggttcccag atgtggccat tggtgcaccc aaggaggatg
     3421 acttcgcagg ggcggtctat atctatcatg gtgatgccgg tgggatagtc cctcagtact
     3481 caatgaaact gtctgggcag aagataaatc cagtgctccg gatgtttggt cagtccatat
     3541 cgggaggcat tgatatggat ggaaatggct atcctgatgt cactgttgga gccttcatgt
     3601 ccgacagcgt ggttcttctc agagcaaggc ctgtcattac ggtggatgtc tccatcttcc
     3661 tcccgggctc catcaacatc acagcgcctc agtgtcacga cggacagcag cctgtgaact
     3721 gcctgaacgt caccacctgc ttcagcttcc atggcaaaca cgttccagaa gagattggcc
     3781 tgaattatgt tctgatggct gacgtggcca aaaaggagaa gggccagatg cccagggtct
     3841 actttgtgct gctgggagag accatgggtc aggtcacaga gaagctgcag ctgacttaca
     3901 tggaggagac gtgtcgtcac tatgtggccc atgtgaagcg gagggtgcag gacgtcatca
     3961 gcccgatcgt gtttgaagca gcctacagcc tcagtgagca tgtgactgga gaggaggaga
     4021 gggaactgcc gcctctgaca ccagttctcc gctggaaaaa gggacaaaag attgcccaaa
     4081 agaatcagac tgtttttgaa aggaattgcc gttcagagga ctgtgccgca gacctgcagc
     4141 ttcagggtaa actgctgctc tccagtatgg atgagaaaac cctgtatcta gctttggggg
     4201 ctgtgaagaa catctcccta aacatctcta tctccaacct cggagatgat gcctatgatg
     4261 ccaacgtgtc cttcaatgtt tcccgggagc tcttcttcat caacatgtgg cagaaggagg
     4321 agatgggcat ctcctgtgag ctgctggaat cggacttcct caaatgcagc gtgggatttc
     4381 ctttcatgag gtcaaagtca aagtatgaat tcagcgtgat ctttgataca agccacctgt
     4441 ctggggaaga ggaagttctc agcttcattg ttactgctca gagtggcaac acggagcgct
     4501 ctgaatccct gcatgacaac accctcgtgc tgatggtgcc actgatgcac gaggtggaca
     4561 cgtccatcac cggaatcatg tctccaacct cctttgtata tggcgagtcc gtggacgcag
     4621 ccaacttcat tcagctggat gacctggagt gtcactttca gcccatcaat atcacccttc
     4681 aggtctacaa cactggccca agcacccttc cagggtcatc tgtcagcatc tctttcccta
     4741 atcgactctc atctggtggt gcagagatgt ttcatgtcca ggaaatggtg gtgggccaag
     4801 agaagggaaa ctgctctttc cagaaaaacc caactccctg catcatccct caagaacaag
     4861 aaaatatctt ccacacaata tttgcttttt tcacaaagtc tggaagaaaa gtcttggact
     4921 gtgaaaaacc aggaatttct tgcctaacag cacactgtaa ctttagtgct cttgctaaag
     4981 aagaaagtcg tactatagac atttacatgc tgctgaacac agaaatactg aaaaaggaca
     5041 gttcgtctgt catccagttc atgtcccgcg ccaaggtgaa ggtggatcct gccctaaggg
     5101 tggtggaaat agctcatggg aacccagaag aggtgacggt ggtcttcgag gccctgcaca
     5161 atctggagcc ccgtggctac gtcgtggggt ggatcatcgc catcagtttg ttggtgggaa
     5221 tcctcatctt cctgctgctg gccgtactag tctggaagct cggcttcttc aaaagaaaat
     5281 atgaaaagat gaccaaaaat ccagatgaga ttgatgagac cacagagctc agtagctgaa
     5341 ccagcagacc tacctgcagt gggaaccggc agcatcccag ccagggtttg ctgtttgcgt
     5401 gcatggattt cttgcggccg ctcgagcatg catctagagg gccctattct atagtgtcac
     5461 ctaaatgcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     5521 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     5581 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     5641 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     5701 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     5761 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     5821 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     5881 tatacaagaa aattatggaa aaatatttga tgtatagtgc cttgactaga gatcataatc
     5941 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     6001 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     6061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcccgatcc
     6181 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     6241 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     6301 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     6361 tcgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa
     6421 agcggggctt cggttgtacg cggttaggag tcccctcagg atatagtagt ttcgcttttg
     6481 catagggagg gggaaatgta gtcttatgca atacacttgt agtcttgcaa catggtaacg
     6541 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     6601 gtaaggtggt acgatcgtgc cttattagga aggcaacaga caggtctgac atggattgga
     6661 cgaaccactg aattccgcat tgcagagata attgtattta agtgcctagc tcgatacaat
     6721 aaacgccatt tgaccattca ccacattggt gtgcacctcc taagctagct ttgccgcaag
     6781 cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa
     6841 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
     6901 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     6961 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     7021 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     7081 agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     7141 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7201 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     7261 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     7321 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     7381 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     7441 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     7501 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     7561 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     7621 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     7681 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     7741 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     7801 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7861 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     7921 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7981 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8041 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccgggctc gatcccctcg
     8101 cgagttggtt cagctgctgc ctgaggctgg acgacctcgc ggagttctac cggcagtgca
     8161 aatccgtcgg catccaggaa accagcagcg gctatccgcg catccatgcc cccgaactgc
     8221 aggagtgggg aggcacgatg gccgctttgg tcccggatct ttgtgaagga accttacttc
     8281 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     8341 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     8401 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     8461 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     8521 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     8581 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     8641 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     8701 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     8761 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     8821 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     8881 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     8941 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     9001 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9061 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatc ccgccatggt
     9121 atcaacgcca tatttctatt tacagtaggg acctcttcgt tgtgtaggta ccgctgtatt
     9181 cctagggaaa tagtagaggc accttgaact gtctgcatca gccatatagc ccccgctgtt
     9241 cgacttacaa acacaggcac agtactgaca aacccataca cctcctctga aatacccata
     9301 gttgctaggg ctgtctccga actcattaca ccctccaaag tcagagctgt aatttcgcca
     9361 tcaagggcag cgagggcttc tccagataaa atagcttctg ccgagagtcc cgtaagggta
     9421 gacacttcag ctaatccctc gatgaggtct actagaatag tcagtgcggc tcccattttg
     9481 aaaattcact tacttgatca gcttcagaag atggcggagg gcctccaaca cagtaatttt
     9541 cctcccgact cttaaaatag aaaatgtcaa gtcagttaag caggaagtgg actaactgac
     9601 gcagctggcc gtgcgacatc ctcttttaat tagttgctag gcaacgccct ccagagggcg
     9661 tgtggttttg caagaggaag caaaagcctc tccacccagg cctagaatgt ttccacccaa
     9721 tcattactat gacaacagct gtttttttta gtattaagca gaggccgggg acccctgggc
     9781 ccgcttactc tggagaaaaa gaagagaggc attgtagagg cttccagagg caacttgtca
     9841 aaacaggact gcttctattt ctgtcacact gtctggccct gtcacaaggt ccagcacctc
     9901 cataccccct ttaataagca gtttgggaac gggtgcgggt cttactccgc ccatcccgcc
     9961 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10021 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10081 tggaggccta ggcttttgca aaaagctaat tc
//
