LOCUS       sequence-77054-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Rodrigues MA, Gomes DA, Leite MF, Grant W, Zhang L, Lam W, Cheng YC,
            Bennett AM, Nathanson MH
  TITLE     Nucleoplasmic calcium is required for cell proliferation.
  JOURNAL   J Biol Chem. 2007 Jun 8. 282(23):17061-8.
  PUBMED    17420246
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jun 8. 282(23):17061-8."
COMMENT     Sequence Label: pcDNA5-TO-PV-NLS-Dsred
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..50)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(63..83)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(87..107)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(111..131)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(144..818)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(203..226)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     618..638
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ctagactatg cggccccatt cagatcctct tctgagatga gtttttgttc tgcggccgta
       61 tctacctttc tcttcttttt tggatctacc tttctcttct tttttggatc tacctttctc
      121 ttcttttttg gatctgcggc cgccaggaac aggtggtggc ggccctcggt gcgctcgtac
      181 tgctccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggc gtccacgtag
      241 tagtagccgg gcagctgcac gggcttcttg gccatgtaga tggacttgaa ctccaccagg
      301 tagtggccgc cgtccttcag cttcagggcc ttgtgggtct cgcccttcag cacgccgtcg
      361 cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg catcacgggg
      421 ccgtcggagg ggaagttcac gccgatgaac ttcaccttgt agatgaagca gccgtcctgc
      481 agggaggagt cctgggtcac ggtcgccacg ccgccgtcct cgaagttcat cacgcgctcc
      541 cacttgaagc cctcggggaa ggacagcttc ttgtagtcgg ggatgtcggc ggggtgcttc
      601 acgtacacct tggagccgta ctggaactgg ggggacagga tgtcccaggc gaagggcagg
      661 gggccgccct tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg gcggccctcg
      721 ccctcgccct cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg caccttgaag
      781 cgcatgaact cggtgatgac gttctcggag gaggccattg cggccgcgct ttcggccacc
      841 agagtggaga attcttc
//
