LOCUS       sequence-77055-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Pusl T, Wu JJ, Zimmerman TL, Zhang L, Ehrlich BE, Berchtold MW, Hoek
            JB, Karpen SJ, Nathanson MH, Bennett AM
  TITLE     Epidermal growth factor-mediated activation of the ETS domain 
            transcription factor Elk-1 requires nuclear calcium.
  JOURNAL   J Biol Chem. 2002 Jul 26. 277(30):27517-27.
  PUBMED    11971908
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Jul 26. 277(30):27517-27."
COMMENT     Sequence Label: pCMV-PV-NLS-GFP
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..32)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(45..65)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(69..89)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(93..113)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(126..836,837..839,840..842))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(166..186)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     781..809
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttcagatcct cttctgagat gagtttttgt tctgcggccg tatctacctt tctcttcttt
       61 tttggatcta cctttctctt cttttttgga tctacctttc tcttcttttt tggatctgcg
      121 gccgctttgt agagctcatc catgccatgt gtaatcccag cagcagttac aaactcaaga
      181 aggaccatgt ggtcacgctt ttcgttggga tctttcgaaa gggcagattg tgtcgacagg
      241 taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg ttgataatgg
      301 tctgctagtt gaacggatcc atcttcaatg ttgtggcgaa ttttgaagtt agctttgatt
      361 ccattctttt gtttgtctgc cgtgatgtat acattgtgtg agttatagtt gtactcgagt
      421 ttgtgtccga gaatgtttcc atcttcttta aaatcaatac cttttaactc gatacgatta
      481 acaagggtat caccttcaaa cttgacttca gcacgcgtct tgtagttccc gtcatctttg
      541 aaagatatag tgcgttcctg tacataacct tcgggcatgg cactcttgaa aaagtcatgc
      601 cgtttcatat gatccggata acgggaaaag cattgaacac cataagagaa agtagtgaca
      661 agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag ggtaagcttt
      721 ccgtatgtag catcaccttc accctctcca ctgacagaaa atttgtgccc attaacatca
      781 ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc tttgctagcc
      841 attgcggccg cgctttcggc caccagagtg gagaattctt caaccccaat cttgccgtcc
      901 ccgtccttgt ctccagcagc catcagcgtc tttgtttcct tagcagacaa gtctctggca
      961 tctgag
//
