LOCUS       sequence-77057-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FLAG tagged Ago1 in S2 cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Kawamata T, Seitz H, Tomari Y
  TITLE     Structural determinants of miRNAs for RISC loading and 
            slicer-independent unwinding.
  JOURNAL   Nat Struct Mol Biol. 2009 Sep;16(9):953-60. doi: 10.1038/nsmb.1630. 
            Epub 2009 Aug 16.
  PUBMED    19684602
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.1630";
            journalName: "Nat Struct Mol Biol"; date: "2009-09"; volume: "16"; 
            issue: "9"; pages: "953-60"
COMMENT     Sequence Label: pAFW-Ago1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..176
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    186..210
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccagatatcg aggcctgtct agagaagctt gttcgaatct cgagtgcgcg cttccggagg
       61 tatacaccta ggcggtacca ctgcagtgaa ttcggagctc cgccaccatg gactacaaag
      121 accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagcacc
      181 ggtatacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgtatccag
      241 ttggacaaca gtcacagtgg accccctcgc ccactcggcc tcagagtccc tctcaggcgc
      301 agactagctt cgacacgctc acatcaccac cagcgcccgg ctcgtcggtc aatcccaccg
      361 ccgtcaccag cccaagtgcc cagaatgtgg ccgctggtgg agcaactgtg gccggtgcag
      421 cagcaactgc cgcccaggtg gcctccgcct tgggtgccac caccggtagc gtgacgccag
      481 caattgccac cgcaacgcca gccacgcagc cggatatgcc cgtctttacg tgtccacgac
      541 gcccgaatct cggacgagag ggtcgcccga ttgtgctgcg cgccaatcac ttccaggtga
      601 caatgccgcg tggctatgtg catcactatg acatcaatat acagccggac aagtgtccgc
      661 gaaaggtgaa ccgtgagatt atcgagacta tggtgcatgc ctatagcaag atcttcggag
      721 tgcttaagcc tgtgttcgat ggtcgcaaca atctgtatac ccgcgatccc ctgcccattg
      781 gcaacgagcg tttggagctg gaggttactc tacccggcga gggcaa
//
