LOCUS       sequence-77058-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Pusl T, Wu JJ, Zimmerman TL, Zhang L, Ehrlich BE, Berchtold MW, Hoek
            JB, Karpen SJ, Nathanson MH, Bennett AM
  TITLE     Epidermal growth factor-mediated activation of the ETS domain 
            transcription factor Elk-1 requires nuclear calcium.
  JOURNAL   J Biol Chem. 2002 Jul 26. 277(30):27517-27.
  PUBMED    11971908
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Jul 26. 277(30):27517-27."
COMMENT     Sequence Label: pCMV-PV-NES-GFP
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..35)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(45..755,756..758,759..761))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(85..105)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     700..728
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccattcagat cctcttctga gatgagtttt tgttctgcgg ccgctttgta gagctcatcc
       61 atgccatgtg taatcccagc agcagttaca aactcaagaa ggaccatgtg gtcacgcttt
      121 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg
      181 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacggatcca
      241 tcttcaatgt tgtggcgaat tttgaagtta gctttgattc cattcttttg tttgtctgcc
      301 gtgatgtata cattgtgtga gttatagttg tactcgagtt tgtgtccgag aatgtttcca
      361 tcttctttaa aatcaatacc ttttaactcg atacgattaa caagggtatc accttcaaac
      421 ttgacttcag cacgcgtctt gtagttcccg tcatctttga aagatatagt gcgttcctgt
      481 acataacctt cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atccggataa
      541 cgggaaaagc attgaacacc ataagagaaa gtagtgacaa gtgttggcca tggaacaggt
      601 agttttccag tagtgcaaat aaatttaagg gtaagctttc cgtatgtagc atcaccttca
      661 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt
      721 gggacaactc cagtgaaaag ttcttctcct ttgctagcca ttgcggccgc gctttcggcc
      781 accagagtgg agaattcttc aaccccaatc ttgccgtccc cgtccttgtc tccagcagcc
      841 atcagcgtct ttgtttcctt agcagacaag tctctggcat ctgaggagaa gcccttcaga
      901 atggacccca gctcatcctc ctcaatgaag ccacttttgt ctttgtccag aatg
//
