LOCUS       sequence-7706-ex       10062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Young BA, Taooka Y, Liu S, Askins KJ, Yokosaki Y, Thomas SM, 
            Sheppard D
  TITLE     The cytoplasmic domain of the integrin alpha9 subunit requires the 
            adaptor protein paxillin to inhibit cell spreading but promotes cell
            migration in a paxillin-independent manner.
  JOURNAL   Mol Biol Cell. 2001 Oct . 12(10):3214-25.
  PUBMED    11598204
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2001 Oct . 12(10):3214-25."
COMMENT     Sequence Label: integrin alpha9 / alpha4 pcDNA1 neo
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            join(1204..1278,1279..1363,1364..1383)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     enhancer        1542..1921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1922..2125
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2076..2096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2170..2189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2170..2188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5398..5416)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5399..5416)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          5500..5565
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5695..5715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5987..6121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6149..6168)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6445..6707
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             7067..7861
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7121..7140)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7731..7750
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          8282..8347
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8477..8497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8922..9056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9903..10038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     1201 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     1261 agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     1321 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     1381 aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     1441 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     1501 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     1561 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     1621 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1681 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1741 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1801 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1861 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1921 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1981 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2041 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2101 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     2161 tatcgaaatt aatacgactc actataggga gacccaagct tagagcgggc gctatgggga
     2221 gccggacgcc agagtcccct ctccatgccg tgcagctgcg ctggggcccc cggcgccgac
     2281 ccccgctgct gccgctgcta ttgctgctcg tgccgccgcc accgcgggtc gggggctaca
     2341 acctggatcc gcagcgcccc gtgcacttcc aaggccccgc tgactcgttc ttcggctacg
     2401 cagttctgga gcatttccac gacaacacgc gctgggtcct tgtgggcgca ccaaaggcag
     2461 attccaaata cagcccttca gtgaagtctc ctggggctgt gtttaagtgc cgtgttcaca
     2521 ccaaccctga ccggagatgc accgaactgg acatggctcg agggaagaat cggggcacgt
     2581 cctgcggaaa gacctgccgg gaagaccgcg atgatgagtg gatgggggtg agcctggccc
     2641 gacagcccaa ggctgatggc cgtgtgttgg cctgtgctca tcgctggaag aacatctact
     2701 atgaagccga ccacatccta ccccatggct tctgctacat catcccctcc aacctccagg
     2761 ccaaaggcag gacgctgatc ccttgctatg aagagtataa gaagaagtac ggagaggaac
     2821 acggctcctg ccaggctggg atagcgggct tcttcaccga ggagctggtg gtgatgggtg
     2881 ctccagggtc attttattgg gctggaacca tcaaagtgct gaaccttacg gacaacacct
     2941 atttaaaact gaacgacgaa gtgatcatga acaggcggta cacctacctg ggctacgcag
     3001 tgaccgctgg ccacttctct cacccgtcca ccattgatgt ggtaggaggt gccccacagg
     3061 acaaaggcat cggcaaggtt tatattttca gagctgaccg aagatcaggc accttaatta
     3121 agatctttca agcatcaggt aaaaagatgg gctcttactt cggctcctcc ttgtgcgcag
     3181 ttgacctgaa tggggacggc ctctctgacc tgctggtggg ggcccccatg ttttctgaga
     3241 tcagggatga gggacaggtc actgtctaca tcaacagagg aaatggagcc ctcgaggagc
     3301 agctggctct gactggggat ggtgcctaca atgcccactt tggagagagc attgccagcc
     3361 tggacgatct ggacaatgat gggttcccag atgtggccat tggtgcaccc aaggaggatg
     3421 acttcgcagg ggcggtctat atctatcatg gtgatgccgg tgggatagtc cctcagtact
     3481 caatgaaact gtctgggcag aagataaatc cagtgctccg gatgtttggt cagtccatat
     3541 cgggaggcat tgatatggat ggaaatggct atcctgatgt cactgttgga gccttcatgt
     3601 ccgacagcgt ggttcttctc agagcaaggc ctgtcattac ggtggatgtc tccatcttcc
     3661 tcccgggctc catcaacatc acagcgcctc agtgtcacga cggacagcag cctgtgaact
     3721 gcctgaacgt caccacctgc ttcagcttcc atggcaaaca cgttccagaa gagattggcc
     3781 tgaattatgt tctgatggct gacgtggcca aaaaggagaa gggccagatg cccagggtct
     3841 actttgtgct gctgggagag accatgggtc aggtcacaga gaagctgcag ctgacttaca
     3901 tggaggagac gtgtcgtcac tatgtggccc atgtgaagcg gagggtgcag gacgtcatca
     3961 gcccgatcgt gtttgaagca gcctacagcc tcagtgagca tgtgactgga gaggaggaga
     4021 gggaactgcc gcctctgaca ccagttctcc gctggaaaaa gggacaaaag attgcccaaa
     4081 agaatcagac tgtttttgaa aggaattgcc gttcagagga ctgtgccgca gacctgcagc
     4141 ttcagggtaa actgctgctc tccagtatgg atgagaaaac cctgtatcta gctttggggg
     4201 ctgtgaagaa catctcccta aacatctcta tctccaacct cggagatgat gcctatgatg
     4261 ccaacgtgtc cttcaatgtt tcccgggagc tcttcttcat caacatgtgg cagaaggagg
     4321 agatgggcat ctcctgtgag ctgctggaat cggacttcct caaatgcagc gtgggatttc
     4381 ctttcatgag gtcaaagtca aagtatgaat tcagcgtgat ctttgataca agccacctgt
     4441 ctggggaaga ggaagttctc agcttcattg ttactgctca gagtggcaac acggagcgct
     4501 ctgaatccct gcatgacaac accctcgtgc tgatggtgcc actgatgcac gaggtggaca
     4561 cgtccatcac cggaatcatg tctccaacct cctttgtata tggcgagtcc gtggacgcag
     4621 ccaacttcat tcagctggat gacctggagt gtcactttca gcccatcaat atcacccttc
     4681 aggtctacaa cactggccca agcacccttc cagggtcatc tgtcagcatc tctttcccta
     4741 atcgactctc atctggtggt gcagagatgt ttcatgtcca ggaaatggtg gtgggccaag
     4801 agaagggaaa ctgctctttc cagaaaaacc caactccctg catcatccct caagaacaag
     4861 aaaatatctt ccacacaata tttgcttttt tcacaaagtc tggaagaaaa gtcttggact
     4921 gtgaaaaacc aggaatttct tgcctaacag cacactgtaa ctttagtgct cttgctaaag
     4981 aagaaagtcg tactatagac atttacatgc tgctgaacac agaaatactg aaaaaggaca
     5041 gttcgtctgt catccagttc atgtcccgcg ccaaggtgaa ggtggatcct gccctaaggg
     5101 tggtggaaat agctcatggg aacccagaag aggtgacggt ggtcttcgag gccctgcaca
     5161 atctggagcc ccgtggctac gtcgtggggt ggatcatcgc catcagtttg ttggtgggaa
     5221 tcctcatctt cctgctgctg gccgtactag tctggaaggc tggcttcttt aaaagacaat
     5281 acaaatctat cctacaagaa gaaaacagaa gagacagttg gagttatatc aacagtaaaa
     5341 gcaatgatga ttaataaggt accgcggccg ctcgagcatg catctagagg gccctattct
     5401 atagtgtcac ctaaatgcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca
     5461 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     5521 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     5581 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     5641 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     5701 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     5761 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     5821 gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc cttgactaga
     5881 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5941 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     6001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     6121 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6181 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     6241 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     6301 ttgcgctgct tcgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt
     6361 ttaggcgaaa agcggggctt cggttgtacg cggttaggag tcccctcagg atatagtagt
     6421 ttcgcttttg catagggagg gggaaatgta gtcttatgca atacacttgt agtcttgcaa
     6481 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6541 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga caggtctgac
     6601 atggattgga cgaaccactg aattccgcat tgcagagata attgtattta agtgcctagc
     6661 tcgatacaat aaacgccatt tgaccattca ccacattggt gtgcacctcc taagctagct
     6721 ttgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag
     6781 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     6841 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     6901 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     6961 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     7021 caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     7081 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7141 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7201 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7261 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7321 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7381 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7441 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7501 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7561 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7621 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7681 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7741 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7801 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7861 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7921 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7981 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccgggctc
     8041 gatcccctcg cgagttggtt cagctgctgc ctgaggctgg acgacctcgc ggagttctac
     8101 cggcagtgca aatccgtcgg catccaggaa accagcagcg gctatccgcg catccatgcc
     8161 cccgaactgc aggagtgggg aggcacgatg gccgctttgg tcccggatct ttgtgaagga
     8221 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     8281 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     8341 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     8401 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     8461 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     8521 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     8581 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     8641 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     8701 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     8761 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     8821 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     8881 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     8941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatc
     9061 ccgccatggt atcaacgcca tatttctatt tacagtaggg acctcttcgt tgtgtaggta
     9121 ccgctgtatt cctagggaaa tagtagaggc accttgaact gtctgcatca gccatatagc
     9181 ccccgctgtt cgacttacaa acacaggcac agtactgaca aacccataca cctcctctga
     9241 aatacccata gttgctaggg ctgtctccga actcattaca ccctccaaag tcagagctgt
     9301 aatttcgcca tcaagggcag cgagggcttc tccagataaa atagcttctg ccgagagtcc
     9361 cgtaagggta gacacttcag ctaatccctc gatgaggtct actagaatag tcagtgcggc
     9421 tcccattttg aaaattcact tacttgatca gcttcagaag atggcggagg gcctccaaca
     9481 cagtaatttt cctcccgact cttaaaatag aaaatgtcaa gtcagttaag caggaagtgg
     9541 actaactgac gcagctggcc gtgcgacatc ctcttttaat tagttgctag gcaacgccct
     9601 ccagagggcg tgtggttttg caagaggaag caaaagcctc tccacccagg cctagaatgt
     9661 ttccacccaa tcattactat gacaacagct gtttttttta gtattaagca gaggccgggg
     9721 acccctgggc ccgcttactc tggagaaaaa gaagagaggc attgtagagg cttccagagg
     9781 caacttgtca aaacaggact gcttctattt ctgtcacact gtctggccct gtcacaaggt
     9841 ccagcacctc cataccccct ttaataagca gtttgggaac gggtgcgggt cttactccgc
     9901 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9961 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10021 gaggcttttt tggaggccta ggcttttgca aaaagctaat tc
//
