LOCUS       sequence-77063-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Pusl T, Wu JJ, Zimmerman TL, Zhang L, Ehrlich BE, Berchtold MW, Hoek
            JB, Karpen SJ, Nathanson MH, Bennett AM
  TITLE     Epidermal growth factor-mediated activation of the ETS domain 
            transcription factor Elk-1 requires nuclear calcium.
  JOURNAL   J Biol Chem. 2002 Jul 26. 277(30):27517-27.
  PUBMED    11971908
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Jul 26. 277(30):27517-27."
COMMENT     Sequence Label: pCMV-PV-NLS-CDEF-GFP
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..35)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(48..68)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(72..92)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(96..116)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      482..491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(548..751)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(577..597)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccattcagat cctcttctga gatgagtttt tgttctgcgg ccgtatctac ctttctcttc
       61 ttttttggat ctacctttct cttctttttt ggatctacct ttctcttctt ttttggatct
      121 gcggccgcgc tttcggccac cagagtggac cacacttcaa ccccaatctt gccgtccccg
      181 tccttggctc cagcagccat cagcgtcttt gtttccttag cagacaagtc tctggcatct
      241 gaggagaagc ccttcagaat ggaccccagc acatcctcct caatgaagcc acttttgtct
      301 ttggccagaa tgtggaacac cttcttcaca tcatccgcac tctttttctt caggcccacc
      361 atctggaaga actttttgtg gtcgaaggag tctgcagcag taaaggctcc tatcgccttc
      421 ttgatgtcct cagcgctgag caagtctgtc atcgacatgt cgacctgcag ctgcacctgg
      481 gccatggtgg ccacgtcgta ttaatttcca cgtgccagta agcagtgggt tctctagtta
      541 gccagaaagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
      601 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
      661 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
      721 gcccattgat gtactgccaa aaccgcatca ctatggtaat agcgatgact aaccgtagat
      781 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      841 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      901 caagtgg
//
