LOCUS       sequence-77065-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination Vector for TALEColor-mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NI(A)-mVenus
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..56)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(191..703,704..706,707..709))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     384..403
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     643..664
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
       61 cgcgcttctc gttggggtct ttgctcagct tggactggta gctcaggtag tggttgtcgg
      121 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      181 cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct
      241 tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      301 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      361 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      421 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      481 cggggtagcg ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg
      541 gcacgggcag cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat
      601 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      661 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccata cttccacttc
      721 cctgcaggcc tgccactcga tgtgatgtcc tctcgggaat ccttctgttg gtccgcttaa
      781 tcaatgcagg cgcgtgcggg agccccttct tcaccgcat
//
