LOCUS       sequence-77066-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of luciferase reporter mRNA in S2 cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Fukaya T, Tomari Y
  TITLE     MicroRNAs mediate gene silencing via multiple different pathways in 
            drosophila.
  JOURNAL   Mol Cell. 2012 Dec 28;48(6):825-36. doi: 
            10.1016/j.molcel.2012.09.024. Epub 2012 Nov 1.
  PUBMED    23123195
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.09.024"; journalName: "Mol Cell"; date: 
            "2012-12-28- 28"; volume: "48"; issue: "6"; pages: "825-36"
COMMENT     Sequence Label: pAWH-Rluc-let-7-Poly(A)
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    246..294
                     /note="synthetic polyadenylation signal"
     protein_bind    complement(334..358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      366..375
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             405..494
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     intron          611..676
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ggcgatcgct cgagactata caaggatcta cctcagtcga gactatacaa ggatctacct
       61 cagtcgagac tatacaagga tctacctcag tcgagactat acaaggatct acctcagtcg
      121 agactataca aggatctacc tcagtcgaga ctatacaagg atctacctca gtcgagacta
      181 tacaaggatc tacctcagtc gagactatac aaggatctac ctcagtcgac gcggccgctg
      241 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaggatc
      301 taaatgagtc ttcggacaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggtga
      361 gctccgccac catggatctc caccgcggtg gaggccgcat cttttaccca tacgatgttc
      421 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
      481 ttccagatta cgctgctcat ggcggacacc ggtccacgtg acgtaagcta gcaggatctt
      541 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
      601 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
      661 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
      721 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
      781 tgctgactct caacattcta ctcctccaaa aa
//
