LOCUS       sequence-7708-ex       10098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Young BA, Taooka Y, Liu S, Askins KJ, Yokosaki Y, Thomas SM, 
            Sheppard D
  TITLE     The cytoplasmic domain of the integrin alpha9 subunit requires the 
            adaptor protein paxillin to inhibit cell spreading but promotes cell
            migration in a paxillin-independent manner.
  JOURNAL   Mol Biol Cell. 2001 Oct . 12(10):3214-25.
  PUBMED    11598204
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2001 Oct . 12(10):3214-25."
COMMENT     Sequence Label: integrin alpha9 W999A pcDNA1 neo
FEATURES             Location/Qualifiers
     source          1..10098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            join(1204..1278,1279..1363,1364..1383)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     enhancer        1542..1921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1922..2125
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2076..2096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2170..2189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2170..2188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5382..5398)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5382..5398)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(5434..5452)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5435..5452)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          5536..5601
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5731..5751
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6023..6157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6185..6204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6481..6743
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             7103..7897
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7157..7176)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7767..7786
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          8318..8383
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8513..8533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8958..9092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9939..10074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
     1201 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
     1261 agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
     1321 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
     1381 aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
     1441 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     1501 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     1561 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     1621 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1681 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1741 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1801 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1861 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1921 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1981 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2041 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2101 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     2161 tatcgaaatt aatacgactc actataggga gacccaagct tagagcgggc gctatgggga
     2221 gccggacgcc agagtcccct ctccatgccg tgcagctgcg ctggggcccc cggcgccgac
     2281 ccccgctgct gccgctgcta ttgctgctcg tgccgccgcc accgcgggtc gggggctaca
     2341 acctggatcc gcagcgcccc gtgcacttcc aaggccccgc tgactcgttc ttcggctacg
     2401 cagttctgga gcatttccac gacaacacgc gctgggtcct tgtgggcgca ccaaaggcag
     2461 attccaaata cagcccttca gtgaagtctc ctggggctgt gtttaagtgc cgtgttcaca
     2521 ccaaccctga ccggagatgc accgaactgg acatggctcg agggaagaat cggggcacgt
     2581 cctgcggaaa gacctgccgg gaagaccgcg atgatgagtg gatgggggtg agcctggccc
     2641 gacagcccaa ggctgatggc cgtgtgttgg cctgtgctca tcgctggaag aacatctact
     2701 atgaagccga ccacatccta ccccatggct tctgctacat catcccctcc aacctccagg
     2761 ccaaaggcag gacgctgatc ccttgctatg aagagtataa gaagaagtac ggagaggaac
     2821 acggctcctg ccaggctggg atagcgggct tcttcaccga ggagctggtg gtgatgggtg
     2881 ctccagggtc attttattgg gctggaacca tcaaagtgct gaaccttacg gacaacacct
     2941 atttaaaact gaacgacgaa gtgatcatga acaggcggta cacctacctg ggctacgcag
     3001 tgaccgctgg ccacttctct cacccgtcca ccattgatgt ggtaggaggt gccccacagg
     3061 acaaaggcat cggcaaggtt tatattttca gagctgaccg aagatcaggc accttaatta
     3121 agatctttca agcatcaggt aaaaagatgg gctcttactt cggctcctcc ttgtgcgcag
     3181 ttgacctgaa tggggacggc ctctctgacc tgctggtggg ggcccccatg ttttctgaga
     3241 tcagggatga gggacaggtc actgtctaca tcaacagagg aaatggagcc ctcgaggagc
     3301 agctggctct gactggggat ggtgcctaca atgcccactt tggagagagc attgccagcc
     3361 tggacgatct ggacaatgat gggttcccag atgtggccat tggtgcaccc aaggaggatg
     3421 acttcgcagg ggcggtctat atctatcatg gtgatgccgg tgggatagtc cctcagtact
     3481 caatgaaact gtctgggcag aagataaatc cagtgctccg gatgtttggt cagtccatat
     3541 cgggaggcat tgatatggat ggaaatggct atcctgatgt cactgttgga gccttcatgt
     3601 ccgacagcgt ggttcttctc agagcaaggc ctgtcattac ggtggatgtc tccatcttcc
     3661 tcccgggctc catcaacatc acagcgcctc agtgtcacga cggacagcag cctgtgaact
     3721 gcctgaacgt caccacctgc ttcagcttcc atggcaaaca cgttccagaa gagattggcc
     3781 tgaattatgt tctgatggct gacgtggcca aaaaggagaa gggccagatg cccagggtct
     3841 actttgtgct gctgggagag accatgggtc aggtcacaga gaagctgcag ctgacttaca
     3901 tggaggagac gtgtcgtcac tatgtggccc atgtgaagcg gagggtgcag gacgtcatca
     3961 gcccgatcgt gtttgaagca gcctacagcc tcagtgagca tgtgactgga gaggaggaga
     4021 gggaactgcc gcctctgaca ccagttctcc gctggaaaaa gggacaaaag attgcccaaa
     4081 agaatcagac tgtttttgaa aggaattgcc gttcagagga ctgtgccgca gacctgcagc
     4141 ttcagggtaa actgctgctc tccagtatgg atgagaaaac cctgtatcta gctttggggg
     4201 ctgtgaagaa catctcccta aacatctcta tctccaacct cggagatgat gcctatgatg
     4261 ccaacgtgtc cttcaatgtt tcccgggagc tcttcttcat caacatgtgg cagaaggagg
     4321 agatgggcat ctcctgtgag ctgctggaat cggacttcct caaatgcagc gtgggatttc
     4381 ctttcatgag gtcaaagtca aagtatgaat tcagcgtgat ctttgataca agccacctgt
     4441 ctggggaaga ggaagttctc agcttcattg ttactgctca gagtggcaac acggagcgct
     4501 ctgaatccct gcatgacaac accctcgtgc tgatggtgcc actgatgcac gaggtggaca
     4561 cgtccatcac cggaatcatg tctccaacct cctttgtata tggcgagtcc gtggacgcag
     4621 ccaacttcat tcagctggat gacctggagt gtcactttca gcccatcaat atcacccttc
     4681 aggtctacaa cactggccca agcacccttc cagggtcatc tgtcagcatc tctttcccta
     4741 atcgactctc atctggtggt gcagagatgt ttcatgtcca ggaaatggtg gtgggccaag
     4801 agaagggaaa ctgctctttc cagaaaaacc caactccctg catcatccct caagaacaag
     4861 aaaatatctt ccacacaata tttgcttttt tcacaaagtc tggaagaaaa gtcttggact
     4921 gtgaaaaacc aggaatttct tgcctaacag cacactgtaa ctttagtgct cttgctaaag
     4981 aagaaagtcg tactatagac atttacatgc tgctgaacac agaaatactg aaaaaggaca
     5041 gttcgtctgt catccagttc atgtcccgcg ccaaggtgaa ggtggatcct gccctaaggg
     5101 tggtggaaat agctcatggg aacccagaag aggtgacggt ggtcttcgag gccctgcaca
     5161 atctggagcc ccgtggctac gtcgtggggt ggatcatcgc catcagtttg ttggtgggaa
     5221 tcctcatctt cctgctgctg gccgtgctgc tctggaagat gggcttcttt cgccgaaggt
     5281 acaaagaaat tatcgaagct gagaagaacc ggaaagagaa tgaagacagt gcggactggg
     5341 tccagaaaaa ccagtgagct gccacaccag tcacatgacc tgatccacta gttctagagc
     5401 ggccgcctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagg
     5461 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     5521 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     5581 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     5641 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     5701 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     5761 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     5821 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     5881 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     5941 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     6001 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     6061 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6121 gtccaaactc atcaatgtat cttatcatgt ctggatctcc cgatccccta tggtgcactc
     6181 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     6241 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     6301 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     6361 ccagatatac gcgtatctga ggggactagg gtgtgtttag gcgaaaagcg gggcttcggt
     6421 tgtacgcggt taggagtccc ctcaggatat agtagtttcg cttttgcata gggaggggga
     6481 aatgtagtct tatgcaatac acttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6541 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6601 tcgtgcctta ttaggaaggc aacagacagg tctgacatgg attggacgaa ccactgaatt
     6661 ccgcattgca gagataattg tatttaagtg cctagctcga tacaataaac gccatttgac
     6721 cattcaccac attggtgtgc acctcctaag ctagctttgc cgcaagcact cagggcgcaa
     6781 gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg
     6841 gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca
     6901 ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag
     6961 cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa
     7021 ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga
     7081 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     7141 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     7201 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     7261 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     7321 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7381 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     7441 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     7501 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     7561 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     7621 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     7681 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     7741 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     7801 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     7861 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     7921 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     7981 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8041 gatctcatgc tggagttctt cgcccacccc gggctcgatc ccctcgcgag ttggttcagc
     8101 tgctgcctga ggctggacga cctcgcggag ttctaccggc agtgcaaatc cgtcggcatc
     8161 caggaaacca gcagcggcta tccgcgcatc catgcccccg aactgcagga gtggggaggc
     8221 acgatggccg ctttggtccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     8281 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     8341 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     8401 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     8461 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     8521 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     8581 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     8641 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     8701 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     8761 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     8821 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     8881 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     8941 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9061 aactcatcaa tgtatcttat catgtctgga tcgatcccgc catggtatca acgccatatt
     9121 tctatttaca gtagggacct cttcgttgtg taggtaccgc tgtattccta gggaaatagt
     9181 agaggcacct tgaactgtct gcatcagcca tatagccccc gctgttcgac ttacaaacac
     9241 aggcacagta ctgacaaacc catacacctc ctctgaaata cccatagttg ctagggctgt
     9301 ctccgaactc attacaccct ccaaagtcag agctgtaatt tcgccatcaa gggcagcgag
     9361 ggcttctcca gataaaatag cttctgccga gagtcccgta agggtagaca cttcagctaa
     9421 tccctcgatg aggtctacta gaatagtcag tgcggctccc attttgaaaa ttcacttact
     9481 tgatcagctt cagaagatgg cggagggcct ccaacacagt aattttcctc ccgactctta
     9541 aaatagaaaa tgtcaagtca gttaagcagg aagtggacta actgacgcag ctggccgtgc
     9601 gacatcctct tttaattagt tgctaggcaa cgccctccag agggcgtgtg gttttgcaag
     9661 aggaagcaaa agcctctcca cccaggccta gaatgtttcc acccaatcat tactatgaca
     9721 acagctgttt tttttagtat taagcagagg ccggggaccc ctgggcccgc ttactctgga
     9781 gaaaaagaag agaggcattg tagaggcttc cagaggcaac ttgtcaaaac aggactgctt
     9841 ctatttctgt cacactgtct ggccctgtca caaggtccag cacctccata ccccctttaa
     9901 taagcagttt gggaacgggt gcgggtctta ctccgcccat cccgccccta actccgccca
     9961 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    10081 tttgcaaaaa gctaattc
//
