LOCUS       sequence-77087-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of TRBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Takahashi T, Miyakawa T, Zenno S, Nishi K, Tanokura M, Ui-Tei K
  TITLE     Distinguishable in vitro binding mode of monomeric TRBP and dimeric 
            PACT with siRNA.
  JOURNAL   PLoS One. 2013 May 2;8(5):e63434. doi: 10.1371/journal.pone.0063434.
            Print 2013.
  PUBMED    23658827
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-2"; volume: "8(5):e63434. doi"; pages: " 
            10.1371/journal.pone.0063434. Print 2013"
COMMENT     Sequence Label: pET28a-TRBP
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    46..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             85..107
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             126..143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             153..170
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
       61 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca
      121 gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggcta
      181 gcatgagtga agaggagcaa ggctccggca ctaccacggg ctgcgggctg cctagtatag
      241 agcaaatgct ggccgccaac ccaggcaaga ccccgatcag ccttctgcag gagtatggga
      301 ccagaatagg gaagacgcct gtgtacgacc ttctcaaagc cgagggccaa gcccaccagc
      361 ctaatttcac cttccgggtc accgttggcg acaccagctg cactggtcag ggccccagca
      421 agaaggcagc caagcacaag gcagctgagg tggccctcaa acacctcaaa ggggggagca
      481 tgctggagcc ggccctggag gacagcagtt ctttttctcc cctagactct tcactgcctg
      541 aggacattcc ggtttttact gctgcagcag ctgctacccc agttccatct gtagtcctaa
      601 ccaggagccc ccccatggaa ctgcagcccc ctgtctcccc tcagcagtct gagtgcaacc
      661 ccgttggtgc tctgcaggag ctggtggtgc agaaaggctg gcggttgccg gagtacacag
      721 tgacccagga gtctgggcca gcccaccgca aagaattcac catgacctgt cgagtggagc
      781 gtttcattga gattgggagt ggcacttcca aaaaattggc aaagcggaat gcggcggcca
      841 aaatgctgct tcgagtgcac acggtgcctc tggatgcccg ggatggcaat gag
//
