LOCUS       sequence-77089-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PACT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Takahashi T, Miyakawa T, Zenno S, Nishi K, Tanokura M, Ui-Tei K
  TITLE     Distinguishable in vitro binding mode of monomeric TRBP and dimeric 
            PACT with siRNA.
  JOURNAL   PLoS One. 2013 May 2;8(5):e63434. doi: 10.1371/journal.pone.0063434.
            Print 2013.
  PUBMED    23658827
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-2"; volume: "8(5):e63434. doi"; pages: " 
            10.1371/journal.pone.0063434. Print 2013"
COMMENT     Sequence Label: pET28a-PACT
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             150..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             177..194
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcctgg
      181 tgccgcgcgg cagccatatg gctagcggat ccatgtccca gagcaggcac cgcgccgagg
      241 ccccgccgct ggagcgcgag gacagtggga ccttcagttt ggggaagatg ataacagcta
      301 agccagggaa aacaccgatt caggtattac acgaatacgg catgaagacc aagaacatcc
      361 cagtttatga atgtgaaaga tctgatgtgc aaatacacgt gcccactttc accttcagag
      421 taaccgttgg tgacataacc tgcacaggtg aaggtacaag taagaagctg gcgaaacata
      481 gagctgcaga ggctgccata aacattttga aagccaatgc aagtatttgc tttgcagttc
      541 ctgacccctt aatgcctgac ccttccaagc aaccaaagaa ccagcttaat cctattggtt
      601 cattacagga attggctatt catcatggct ggagacttcc tgaatatacc ctttcccagg
      661 agggaggacc tgctcataag agagaatata ctacaatttg caggctagag tcatttatgg
      721 aaactggaaa gggggcatca aaaaagcaag ccaaaaggaa tgctgctgag aaatttcttg
      781 ccaaatttag taatatttct ccagagaacc acatttcttt aacaaatgta gtaggacatt
      841 ctttaggatg tacttggcat tccttgagga attctcctgg tgaaaagatc aacttactga
      901 aaagaagcct ccttagtatt ccaaatacag attac
//
