LOCUS       sequence-77099-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and
            contains attL4 and attL1 sites for recombination into a Gateway 
            destination vector at the attR4 and attR1 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pEN41A-T38S38
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..28
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12..28
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    68..163
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccgagttaa cgctaccatg
       61 gagctccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tatagaaaag ttgcagctgg ctagcgagtc
      181 atgaggtcac catggtcatt aggagccgct ctcgcacttc agctgcttct ccaagccgca
      241 gatgatcagc tccaggccga acaagaaggc cggctcggcg ccctggtggt cgaacagctc
      301 gatggcctgg cgcagcaacg gcggcatgct gtcggtggtc ggggtctcgc gctcctcctt
      361 ggcggccgtg tgctcctggt cctccaacac gcagcccagg gtgaagtggc ccacggccga
      421 cagggcgtac aacgcgttct ccagcgagaa gccgttctcg gtcatgaagc gcagctgggt
      481 ctccacggtg tcgtactgct tctcgtccgg gcgggtgccc aggtgcacct tggcgccgtc
      541 gcggtagcgc agcagcgcgc ggcggaacga catggcgttg ttgcgcagga acgactgcca
      601 cgactcgccg gccgccggca gcgagtagtc gtggtggcgc gccaggatct ccaccgccag
      661 ggcgtccagc agggcccgct tgttcttgac gtgccagtac agggtcttct gcgcgacgcc
      721 cagcttctgg gccagcttgc gggtggtcac gccctcgatg ccgacctcgt tgcccagggc
      781 cagggcggag ttgatgacct tggacttgtc caggcgggac attgcgaagt gattcctcct
      841 gatggtaccc taggtcagat caacggcctg gtcgacaa
//
