LOCUS       sequence-77100-e         352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 
            and attL2 sites for recombination into a Gateway destination vector 
            at the attR4 and attR1 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pEN12A-P750
FEATURES             Location/Qualifiers
     source          1..352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(253..352)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tgctaccagg cctagatctg gggaccctag aggtcccctt
      121 ttttatttta aaaatttttt cacgtgccag ccgattggtc gctctgacca gggaaaatag
      181 ccctctgacc tggggatttg cgatccccgt cagtgacgga gataattgtg agcgctcaca
      241 accgcatgca ccacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
      301 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tg
//
