LOCUS       sequence-77104-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat13
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..189
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             205..222
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatgaaagaa accgctgctg ctaaattcga acgccagcac
      181 atggacagcc cagatctggg taccctggtg ccacgcggtt ccatggcgat atcggatcgg
      241 gaaggtgtga atgataatga agaaggcttt ttctctgctc gtgctgctat tgacgctgct
      301 aaccgtgaag aaacgttcgg cccgctggcg ccgctgtttc gtagcattgc aggcggtttc
      361 ccgctggctg gcgttaccgg tcgtatcctg gtcccgctga cgatcgaaga cgcacagatt
      421 cgcgcagatg tggctccgag caacctggct atcgtgggcc gttacgttct gtctgcagac
      481 atttggccgc tgctggctaa actgaccgat atcctggcgg ccggtctgca gcaaaaggca
      541 gaagcagctg aaatcaatct gcgttatcag ggcattccgg ttggcggtta caccaaattt
      601 ggtgcgctgg aatatcgctt tttcagtatc gacggcacga aggcggatct gggtattgcc
      661 ttcgatggcg acggtgatcg tcatccggac accctgctgg cagcgcgtgg cgatcagtac
      721 ccgattgcac tggaaggtgc tctgaaaggc ggttattttc cggttccgcc ggtcgacagc
      781 gcgcaagata ttcgtatccc ggtggttagc tctccgaaaa gtggcaccgg ttccgtggac
      841 tacgcaaagg ctgttggcga ttcactggaa gcgcagcaat atggtatcgc ctttcc
//
