LOCUS       sequence-77130-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRa11_3726
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             849..866
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(919..937)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      933..980
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgcgtat taccgcaagc ctgcgtacct
      241 tttgtcatct gagcacaccg catagcgata gcaccaccag tcgtctgtgg attgatgaag
      301 ttaccgcagt tgcacgtcag cgtgatcgtg atagctttat gcgcatctat gatcattttg
      361 caccgcgtct gctgcgttat ctgaccggtc tgaatgttcc ggaaggtcag gcagaagaac
      421 tggttcaaga ggttctgctg aaactgtggc ataaagcaga aagttttgat ccgagcaaag
      481 caagcctggg cacctggctg tttcgtattg cacgtaatct gtatattgac agcgtgcgta
      541 aagatcgtgg ttgggttcag gttcagaata gcctggaaca gctggaacgt ctggaagcac
      601 cggttgatcg taccctggat tatagccagc gtcaagaaca gcagctgaat agtgcaattc
      661 agaatctgcc gaccgatcag gcaaaagttc tgcgtatgag ctattttgaa gcactgagcc
      721 atcgtgaaat tagcgaacgc ctggatatgc cgctgggcac cgttaaaagc tgtctgcgtc
      781 tggcatttca gaaactgcgt agccgtattg aagaaagcta aggatccaag cttgcggccg
      841 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      901 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      961 gggtcttgag gggttttttg ct
//
