LOCUS       sequence-77134-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRa12_808
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             882..899
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgctgga ttttcgtcag ccgaatcgta
      241 ccaaacaaga atttgaagaa ctggcactgg cacatctgga cccgctgtat agcgcagcac
      301 tgcgtctgac caaaaatgaa cgtgatgcag aagatctggt tcaggatacc tgtatgcgtg
      361 catatcgctt tttcgataaa tttgaacgtg gcaccaacat taaagcctgg ctgtttaaaa
      421 ttctgaccaa caccttcatt aaccgctatc gtcgtaaagt taaagaacgt accgttgttg
      481 aaggtgtgga acgtgaagca gtgcatgaac gttttgttag ccgtgatgca accgattttg
      541 cagcaaatcc ggaacagtat ttttttgatc gtctgctgag tgatgatgtt ctgcgtgcaa
      601 ttgatgcact gccgattgat tttcgcctgg ttgttattct ggccgatctg caagagttta
      661 gctataaaga aatcgccgag attctggaat gtccggttgg caccgttatg agccgtctgt
      721 ttcgtggtcg taaactgctg cagaaaaatc tgcgtgaata tgccgaaggt cagggtgttt
      781 ttcgtcatga tggtgaaccg gttaatgcac cggcagatct ggaagaatat cgtcatcgta
      841 aaaaaaccgg ctaaggatcc aagcttgcgg ccgcactcga gcaccaccac caccaccact
      901 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctga
//
