LOCUS       sequence-77137-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRa14_1324
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgaatga taccgcagca gatcatagcc
      241 atgcagatgg tccgaccacc ggtcatgcac ataccgcaac ctttagcagt gatgccgatg
      301 gtcaggcatg gacccctccg acctgggaag aaattgttag cacccatagc ggtcgtgttt
      361 atcgtctggc atatcgtctg accggtaatc agcatgatgc agaagatctg acccaagaag
      421 tttttgttcg cgtttttcgt agcctgagca cctatacacc gggtacattt gaaggttggc
      481 tgcatcgtat taccaccaac ctgtttctgg atatggttcg tcgtaaacag cgtattcgtt
      541 ttgatgcact gggtgatgat gcagcagaac gtctgccgag ccgtgaaccg actccgcagc
      601 agctgtttca tgatgcacat tttgatgcgg atgttcagca ggcactggat accctggcac
      661 cggaatttcg tgcagcagtt gttctgtgtg atattgaagg tctgtcctat gaagaaatcg
      721 cagcaaccct gggtgttaaa ctgggcaccg ttcgtagccg tattcatcgt ggtcgtagcc
      781 agctgcgtaa agcactggca catcgtagcc cgaaagcacg tgcagatcgt cgtagtctgg
      841 cagccggtg
//
