LOCUS       sequence-77146-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEColor-mVenus-Telomere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-TelL20-mVenus
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      69..78
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             78..143
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             150..170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accgggaccg atccagcctg gcgcgccggt tctggatccg gttctcaatt gggttctacc
       61 ggttaggggc caccatggac tataaggacc acgacggaga ctacaaggat catgatattg
      121 attacaaaga cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg
      181 gagtcccagc agccgtagat ttgagaactt tgggatattc acagcagcag caggaaaaga
      241 tcaagcccaa agtgaggtcg acagtcgcgc agcatcacga agcgctggtg ggtcatgggt
      301 ttacacatgc ccacatcgta gccttgtcgc agcaccctgc agcccttggc acggtcgccg
      361 tcaagtacca ggacatgatt gcggcgttgc cggaagccac acatgaggcg atcgtcggtg
      421 tggggaaaca gtggagcgga gcccgagcgc ttgaggccct gttgacggtc gcgggagagc
      481 tgagagggcc tccccttcag ctggacacgg gccagttgct gaagatcgcg aagcggggag
      541 gagtcacggc ggtcgaggcg gtgcacgcgt ggcgcaatgc gctcacggga gcacccctca
      601 acctgacccc agagcaggtc gtggcaattg cgagcaacat cgggggaaag caggcactcg
      661 aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cggacttacg ccagagcagg
      721 tcgtggcaat tgcg
//
