LOCUS       sequence-77148-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEColor-mCherry-Telomere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-TelR20-mCherry
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..163
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             170..190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctg gcgcgccggt tctggatccg
       61 gttctcaatt gggttctacc ggttaggggc caccatggac tataaggacc acgacggaga
      121 ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc caaagaagaa
      181 gcggaaggtc ggtatccacg gagtcccagc agccgtagat ttgagaactt tgggatattc
      241 acagcagcag caggaaaaga tcaagcccaa agtgaggtcg acagtcgcgc agcatcacga
      301 agcgctggtg ggtcatgggt ttacacatgc ccacatcgta gccttgtcgc agcaccctgc
      361 agcccttggc acggtcgccg tcaagtacca ggacatgatt gcggcgttgc cggaagccac
      421 acatgaggcg atcgtcggtg tggggaaaca gtggagcgga gcccgagcgc ttgaggccct
      481 gttgacggtc gcgggagagc tgagagggcc tccccttcag ctggacacgg gccagttgct
      541 gaagatcgcg aagcggggag gagtcacggc ggtcgaggcg gtgcacgcgt ggcgcaatgc
      601 gctcacggga gcacccctca acctgacccc agagcaggtc gtggcaattg cgagcaacat
      661 cgggggaaag caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca
      721 cggacttacg ccagagcagg tcgtggcaat tgcgagcaac atcgggggaa agcaggcact
      781 cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacggac
//
