LOCUS       sequence-77149-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEColor-mVenus-Telomere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-TelL15-mVenus
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             86..151
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             158..178
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tagaagacac cgggaccgat ccagcctggc gcgccggttc tggatccggt tctcaattgg
       61 gttctaccgg ttaggggcca ccatggacta taaggaccac gacggagact acaaggatca
      121 tgatattgat tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg
      181 tatccacgga gtcccagcag ccgtagattt gagaactttg ggatattcac agcagcagca
      241 ggaaaagatc aagcccaaag tgaggtcgac agtcgcgcag catcacgaag cgctggtggg
      301 tcatgggttt acacatgccc acatcgtagc cttgtcgcag caccctgcag cccttggcac
      361 ggtcgccgtc aagtaccagg acatgattgc ggcgttgccg gaagccacac atgaggcgat
      421 cgtcggtgtg gggaaacagt ggagcggagc ccgagcgctt gaggccctgt tgacggtcgc
      481 gggagagctg agagggcctc cccttcagct ggacacgggc cagttgctga agatcgcgaa
      541 gcggggagga gtcacggcgg tcgaggcggt gcacgcgtgg cgcaatgcgc tcacgggagc
      601 acccctcaac ctgaccccag agcaggtcgt ggcaattgcg agcaacatcg ggggaaagca
      661 ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg gacttacgcc
      721 agagcaggtc gtggcaattg cgagcaacca cgggggaaag caggcactcg aaaccgtcca
      781 gaggttgctg cctgtgctgt gccaagcgca cggac
//
