LOCUS       sequence-77154-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEColor-mVenus-Chromosome 18-specific Centromere .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-Cen18-mVenus
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..145
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             152..172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acaccgggac cgatccagcc tggcgcgccg gttctggatc cggttctcaa ttgggttcta
       61 ccggttaggg gccaccatgg actataagga ccacgacgga gactacaagg atcatgatat
      121 tgattacaaa gacgatgacg ataagatggc cccaaagaag aagcggaagg tcggtatcca
      181 cggagtccca gcagccgtag atttgagaac tttgggatat tcacagcagc agcaggaaaa
      241 gatcaagccc aaagtgaggt cgacagtcgc gcagcatcac gaagcgctgg tgggtcatgg
      301 gtttacacat gcccacatcg tagccttgtc gcagcaccct gcagcccttg gcacggtcgc
      361 cgtcaagtac caggacatga ttgcggcgtt gccggaagcc acacatgagg cgatcgtcgg
      421 tgtggggaaa cagtggagcg gagcccgagc gcttgaggcc ctgttgacgg tcgcgggaga
      481 gctgagaggg cctccccttc agctggacac gggccagttg ctgaagatcg cgaagcgggg
      541 aggagtcacg gcggtcgagg cggtgcacgc gtggcgcaat gcgctcacgg gagcacccct
      601 caacctgacc ccagagcagg tcgtggcaat tgcgagcaac ggagggggaa agcaggcact
      661 cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacggactta cgccagagca
      721 ggtcgtggca attgcgagca accacggggg aaa
//
