LOCUS       sequence-77155-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEColor-mVenus-Chromosome 15-specific Centromere .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-Cen15-mVenus
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             76..141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgggaccgat ccagcctggc gcgccggttc tggatccggt tctcaattgg gttctaccgg
       61 ttaggggcca ccatggacta taaggaccac gacggagact acaaggatca tgatattgat
      121 tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga
      181 gtcccagcag ccgtagattt gagaactttg ggatattcac agcagcagca ggaaaagatc
      241 aagcccaaag tgaggtcgac agtcgcgcag catcacgaag cgctggtggg tcatgggttt
      301 acacatgccc acatcgtagc cttgtcgcag caccctgcag cccttggcac ggtcgccgtc
      361 aagtaccagg acatgattgc ggcgttgccg gaagccacac atgaggcgat cgtcggtgtg
      421 gggaaacagt ggagcggagc ccgagcgctt gaggccctgt tgacggtcgc gggagagctg
      481 agagggcctc cccttcagct ggacacgggc cagttgctga agatcgcgaa gcggggagga
      541 gtcacggcgg tcgaggcggt gcacgcgtgg cgcaatgcgc tcacgggagc acccctcaac
      601 ctgaccccag agcaggtcgt ggcaattgcg agccatgacg ggggaaagca ggcactcgaa
      661 accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg gacttacgcc agagcaggtc
      721 gtggcaattg cgagcaacat c
//
