LOCUS       sequence-77169-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRb33_375
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     105..123
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(273..301)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     896..916
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
       61 ccgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      121 ggccctttaa gttgaacaat tttgcacctt ccgtcgaacc gtccgctgca tcgacccgac
      181 caaccttgcg agacggcctt tgagcgtgga tcctgtcaat ttccgcgata gaggaggtaa
      241 agatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      301 gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg
      361 gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
      421 ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac
      481 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
      541 tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
      601 ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      661 aactcgagta caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
      721 gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag
      781 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      841 acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
      901 ttcttgagtt tgtaactgct gctgggatta cacatggcat gg
//
