LOCUS       sequence-77179-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Mank M, Santos AF, Direnberger S, Mrsic-Flogel TD, Hofer SB, Stein 
            V, Hendel T, Reiff DF, Levelt C, Borst A, Bonhoeffer T, Hubener M, 
            Griesbeck O
  TITLE     A genetically encoded calcium indicator for chronic in vivo 
            two-photon imaging.
  JOURNAL   Nat Methods. 2008 Sep;5(9):805-11. doi: 10.1038/nmeth.1243.
  PUBMED    19160515
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-09"; volume: "5"; issue: 
            "9"; pages: "805-11"
COMMENT     Sequence Label:  TN-XXL pRSETB
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(19..21,22..24,25..537)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(64..85)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(325..344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(606..624)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      620..667
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggcggtaccg gcggcagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc
      121 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgatgtg cttcgcccgc
      241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      481 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
      541 taagaattcg aagcttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      601 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      661 ttttttgctg aaaggaggaa ctatatccgg atctggcgta atagcgaaga ggcccgcacc
      721 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
      781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagc
//
