LOCUS       sequence-77181-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Mank M, Santos AF, Direnberger S, Mrsic-Flogel TD, Hofer SB, Stein 
            V, Hendel T, Reiff DF, Levelt C, Borst A, Bonhoeffer T, Hubener M, 
            Griesbeck O
  TITLE     A genetically encoded calcium indicator for chronic in vivo 
            two-photon imaging.
  JOURNAL   Nat Methods. 2008 Sep;5(9):805-11. doi: 10.1038/nmeth.1243.
  PUBMED    19160515
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-09"; volume: "5"; issue: 
            "9"; pages: "805-11"
COMMENT     Sequence Label: TN-XXL pcDNA3
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(16..18,19..21,22..534)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(61..82)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(322..341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(601..619)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(602..619)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(639..656)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    645..756
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggtaccggcg gcagcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
       61 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgcgcgg cgagggcgag
      121 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      181 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgatgtgctt cgcccgctac
      241 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      481 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggactaa
      541 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggccctat
      601 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
      661 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      721 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      781 tattctgggg ggtggggtgg ggcaggacag caaggggga
//
