LOCUS       sequence-77185-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc02_915
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             616..627
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aaacagggat ccttaacgat taaccggaat cggggtaact tctgaattat catcaacaac
       61 ggcaccggta ttcagacgct gctgcagcat gtgatcctgc agatatgcat taatacgacg
      121 acgctgttcc agcagctgat catttgcatt accctgattc tgatttgcaa ccggaccggt
      181 ctgcaggcta accggtgttg cggtgccaac cagcggacgg gtattcagaa ccggcagcgg
      241 tgcatcatcc tgggtgctct gataattctg aacaccaaca actgccatca gtgcaactgc
      301 ggctgcaata ccatactgac ccagctgacg cataaacgga acaacaactg cacggctcgg
      361 tgcctgggtt gcttcacgtt caactttcgg tgccagaatg gtcggttcat cttccagtgc
      421 tgctgcaata cgtgcgctca gatccagcgg cattgcctgc ggcagttcat cacgcattgc
      481 atcacccatc agatgataac gctgccattt tgcatggcta tcggtatctg ctgccagttc
      541 tgccagggtc tgacgatcgg tttcaccatc aacgcttgcg ctaacccatt cttgaccggt
      601 tttttccata tggtatttct cctctttctc tagtatttat tcgactataa caaaccattt
      661 tcttgcgtaa acctgtacga tcctacaggt cagccaacga tcgttccgat cagaacaaga
      721 aagctatgtg gcctgacgaa gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg
      781 gcatgatagc gcccggaaga gagtcaattc agggtggtga atatgaaaaa cataaatgcc
      841 gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat
      901 caatgcttat ctgatatgac taaaatgg
//
