LOCUS       sequence-77187-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc03_1198
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             378..389
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aacagggatc cttaattcag gaagatatcg ttttcgctaa agctaaccag ttcttccagt
       61 tcctcatcac tcagttcttt aatccatttg tccaccggat cggtggtaac attgttttta
      121 tcaactgcca gaatattgct tgcgctcggg gtatcggtat tcatataacg caggctcggg
      181 gtaaacagaa tacctgccag aactgctgca actgcaataa cggtcggaat aatacgtttc
      241 agacgacgtt ttttacgctg ttcatcaacg gtacgttcca gaattttgcg ctgggtttct
      301 tcaaaaaagt tatccggtgt acgatacggg gtacgtttac caatattgtc aaagtcgaag
      361 tctttgtcca tatggtattt ctcctctttc tctagtattt attcgactat aacaaaccat
      421 tttcttgcgt aaacctgtac gatcctacag gtcagccaac gatcgttccg atcagaacaa
      481 gaaagctatg tggcctgacg aagcggcgcg ccatcgaatg gcgcaaaacc tttcgcggta
      541 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatatgaaa aacataaatg
      601 ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta
      661 atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca
      721 tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat
      781 ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta
      841 actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat
      901 ctccaaatgt aatta
//
