LOCUS       sequence-77188-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc03_1244
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             430..441
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aaacagggat ccttattcgc taaaatcggt cagaacccat tcatcactaa cggtttcggc
       61 acaattatca tgcagaaaat cacgataatc ggtatcttcg ctccaacggg tatcaccatg
      121 atccagtgca accagacctg cgctggtact ggtggtacga ccggtatcgc taccaacacc
      181 aaacaggcta aaggctttaa acataccaat catcagcaga aagcttgctg ccagatacag
      241 cagcggacgc agtttcggcc acagtgcaac gctcggaaca acaacggtat tttcttcttt
      301 aactgccgga atctgtgcca tcagtttatc ggtgaagctt gcaaaataat catccggaac
      361 gctataatga cgaatgctat cttctttcgg acgaatgtta acatccggat cgaactgttt
      421 catatggtat ttctcctctt tctctagtat ttattcgact ataacaaacc attttcttgc
      481 gtaaacctgt acgatcctac aggtcagcca acgatcgttc cgatcagaac aagaaagcta
      541 tgtggcctga cgaagcggcg cgccatcgaa tggcgcaaaa cctttcgcgg tatggcatga
      601 tagcgcccgg aagagagtca attcagggtg gtgaatatga aaaacataaa tgccgacgac
      661 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc
      721 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct
      781 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa
      841 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat
      901 cattcaccaa ttaatt
//
