LOCUS       sequence-77197-e        4720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for cloning human TRIM9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Short KM, Cox TC
  TITLE     Subclassification of the RBCC/TRIM superfamily reveals a novel motif
            necessary for microtubule binding.
  JOURNAL   J Biol Chem. 2006 Mar 31;281(13):8970-80. Epub 2006 Jan 23.
  PUBMED    16434393
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-03-31"; volume: "281(13)"; pages: "8970-80. Epub
            2006 Jan 23"
COMMENT     Sequence Label: pENTR-D-Topo-hTRIM9
FEATURES             Location/Qualifiers
     source          1..4720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2845..2944)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2961..2980)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2962..2980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2985..3001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2985..3001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3103..3122)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3114..3923
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3181..3200)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4070..4658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4559..4578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat ggaggagatg gaagaggagt tgaaatgccc
      721 cgtgtgcggc tccttctatc gggagcccat catcctgccc tgctctcaca atttgtgtca
      781 ggcgtgcgcc cgcaacatcc tggtgcagac cccagagtct gaatcccccc agagccatcg
      841 ggccgcgggc tccggggtct ccgactatga ctatctggac ctggacaaga tgagcctata
      901 cagcgaggcg gacagcggct atggctccta cggggggttc gccagcgccc ccactacccc
      961 gtgccagaag tcccccaacg gcgtccgcgt gtttcccccg gctatgccgc caccggccac
     1021 ccacttgtca ccggccctgg ccccggtgcc ccgcaactcc tgtatcacct gcccccagtg
     1081 tcaccgcagc ctcatcctgg atgaccgggg gctccgcggc ttccccaaga atcgcgtact
     1141 ggaaggggta attgaccgct accagcagag caaagccgcg gccctcaagt gccagctctg
     1201 cgagaaggcg cccaaggaag ccaccgtcat gtgcgaacag tgcgatgtct tctactgcga
     1261 tccgtgccgc ctgcgctgcc acccgccccg ggggccccta gccaagcacc gcctggtgcc
     1321 cccggcccag ggtcgtgtga gccggaggct gagcccacgc aaggtctcca cctgcacaga
     1381 ccacgagctg gagaaccaca gcatgtactg cgtgcaatgc aagatgcccg tgtgctacca
     1441 gtgcttggag gagggcaaac actccagcca cgaagtcaag gctctggggg ccatgtggaa
     1501 actacataag agccagctct cccaggcgct gaacggactg tcagacaggg ccaaagaagc
     1561 caaggagttt ctggtacagc tgcgcaacat ggtccagcag atccaggaga acagtgtgga
     1621 gtttgaagcc tgtctggtgg cccaatgtga tgccctcatc gatgccctca acagaagaaa
     1681 agcccagctg ctggcccgcg tcaacaagga gcatgagcac aagctgaagg tggttcgaga
     1741 tcagatctct cactgcacag tgaaattgcg ccagaccaca ggtctcatgg agtactgctt
     1801 ggaggtgatt aaggaaaatg atcctagtgg ttttttgcag atttctgacg ccctcataag
     1861 aagagtgcac ctgactgagg atcagtgggg taaaggcaca ctcactccaa ggatgaccac
     1921 ggactttgac ttgagtctgg acaacagccc tctgctgcaa tccatccacc agctggattt
     1981 cgtgcaagtg aaagcttcct ctccagtccc agcaacccct atcctacagc tggaggaatg
     2041 ttgtacccac aacaacagcg ctacgttgtc ctggaaacag ccacctctgt ccacggtgcc
     2101 cgccgatgga tacattctgg agctggatga tggcaacggt ggtcaattcc gggaggtgta
     2161 tgtggggaag gagacaatgt gcactgtgga tggtcttcac ttcaacagca catacaacgc
     2221 tcgggtcaag gccttcaaca aaacaggagt cagcccgtac agcaagaccc tggtcctcca
     2281 aacgtctgag gtggcctggt ttgctttcga ccctggctcg gcgcactcgg acatcatcct
     2341 ctccaatgac aacctgacag tgacctgtag tagctatgat gaccgggtgg tgctagggaa
     2401 gactggcttc tccaagggca tccactactg ggagctcacg gtagatcgct atgacaacca
     2461 ccctgatcct gcctttggtg tggctcgcat ggacgtgatg aaggatgtga tgttaggaaa
     2521 agacgacaaa gcttgggcaa tgtatgtgga caataaccgg agctggttca tgcacaacaa
     2581 ctcgcacacc aacagaactg agggagggat cacaaaaggg gccacaattg gggtcctcct
     2641 cgacttaaat agaaaaaact tgacattttt tatcaacgat gaacaacaag gtcccatagc
     2701 atttgataac gtggagggcc tcttcttccc tgcggtcagc ctgaacagga acgtgcaggt
     2761 cacgctgcac accgggctcc cagtccccga cttctactcc agcagagcat caatagccta
     2821 agaattccaa gggtgggcgc gccgacccag ctttcttgta caaagttggc attataagaa
     2881 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2941 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     3001 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     3061 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     3121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     3181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     3241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     3301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     3361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     3421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     3481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     3541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     3601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     3661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     3721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     3781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     3841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     3901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     3961 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     4021 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4201 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4441 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4681 tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
