LOCUS       sequence-77199-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, 
            Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, 
            Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger
            C, Griesbeck O
  TITLE     Optimized ratiometric calcium sensors for functional in vivo imaging
            of neurons and T lymphocytes.
  JOURNAL   Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773.
  PUBMED    24390440
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773."
COMMENT     Sequence Label: Twitch-1 pcDNA3
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      111..120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(117..119,120..122,123..635)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(162..183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(423..442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     770..791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tcccgcggcc gccaccatgg
      121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      181 acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc accaacggca
      241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      301 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgtacc atcttcttca
      421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      541 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      601 tcaaggccca cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      721 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      781 tggagttcgt gaccgccgcc cgcatgccta gcgaagaaga gctatcagag tgctttcgca
      841 tttttgacaa ggatggaaat ggtttcatcg acagggagga atttggagac atcatccgcc
      901 ttactggaga acagctcaca gatgaggatg tcgatgagat atttggagat tcagacacag
      961 acaaaa
//
